PuroSPIN™ Endo-Free Plasmid MIDIprep Plus Kit #NKE212-20, #NKE212-50

The PuroSPIN™ Endo-Free Plasmid MIDIprep Plus Kit is designed to provide a simple and easy-to-use method for large-scale extraction and purification of endotoxin-free plasmid DNA. This purification technology is based on plasmid binding to silica spin-columns, which allows for efficient removal of genomic DNA, protein, salt, and other contaminants.Following the lysis steps, endotoxins are removed using the Endotoxin Removal Buffer ERB.
 
The PuroSPIN™ Endo-Free Plasmid MIDIprep Plus Kit can extract up to 200 µg of plasmid DNA with endotoxin levels to <0.1 EU/µg. Silica spin columns provide a faster, simpler, and safer alternative to traditional nucleic acid purification techniques such as phenol-chloroform extraction.
 
The PuroSPIN™ buffer formulations produce highly purified plasmid DNA for downstream applications, such as transfection, enzymatic digestion, or PCR.
 
Features:
Purify endotoxin-free plasmid DNA from large volumes of bacterial culture
Extraction of up to 200 µg of plasmid DNA while removing endotoxin to levels of <0.1 EU/µg
"Plus" kit includes a syringe filter that quickly and easily removes insoluble fraction from the plasmid preparation, removing the need for the 12,000 x g centrifugation step
Rapid purification of plasmid DNA (< 60 minutes), compared to ion-exchange column formats (> 3 hours)
Optimized buffer formulations to ensure efficient removal of RNA from plasmid samples
Efficient removal of genomic DNA, protein, salt, and other contaminants
High quality extracted plasmid DNA can be used for a range of downstream applications such as cloning, PCR, restriction enzyme digest, sequencing, transformation, or transfection
 
 
SKU List:
NKE212-20, NKE212-50