PuroSPIN™ DNA/RNA Purification Kit #NK080-50, #NK080-200
The PuroSPIN™ DNA/RNA Purification Kit is designed for simultaneous purification of total DNA and RNA from cultured cells or animal tissue. It greatly simplifies the extraction process by incorporating a convenient and easy-to-use extraction technology based on nucleic acid binding silica spin-columns. Up to 30 mg of animal tissues or 1 x 10^7 eukaryotic cells can be used per experiment with extraction procedure being less than 40 minutes.
Features:
- Easily purify genomic DNA and Total RNA cultured cells and animal tissues using silica spin columns
- Simple extraction process with no need for phenol or chloroform
- High-quality extracted genomic DNA and RNA can be used for a range of downstream applications such as genomic sequencing, PCR, real-time qPCR, or Southern blotting
SKU List: #NK080-5, #NK080-50, #NK080-200
Product Manuals
Materials Safety Data Sheets (MSDS)
Lysis Buffer LB-DR - MSDS
Wash Buffer WB-GD1 - MSDS
Wash Buffer WB-GD2 - MSDS
Wash Buffer WB-TR1 - MSDS
Wash Buffer WB-R2 - MSDS
Elution Buffer EB- MSDS
Nuclease-Free Water - MSDS
Product Data Sheet
Product Specifications
Kit Type: DNA/RNA Extraction/Purification Kit Format: Silica Spin Columns Kit Scale: MINI Starting Material: Up to 1X10^7 cells; up to 30 mg tissue Processing Time: < 40 minutes Typical Yield up to 100 µg for RNA and DNA Elution Volume: 50 - 100 μL Typical Ratio A260/A280: 1.7 - 1.9 for DNA; 2.0 - 2.2 for RNA Typical Ratio A260/A230: 2.0 - 2.3 for RNA and DNA Downstream Applications: Ligation, microarray, qPCR, RT-qPCR, Northern blot, poly-A purification, etc.