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PuroSPIN™  DNA/RNA Purification Kit  #NK080-50, #NK080-200

PuroSPIN™ DNA/RNA Purification Kit #NK080-50, #NK080-200

The PuroSPIN™ DNA/RNA Purification Kit is designed for simultaneous purification of total DNA and RNA from cultured cells or animal tissue. It greatly simplifies the extraction process by incorporating a convenient and easy-to-use extraction technology based on nucleic acid binding silica spin-columns. Up to 30 mg of animal tissues or 1 x 10^7 eukaryotic cells can be used per experiment with extraction procedure being less than 40 minutes.

 

Features:

 

  • Easily purify genomic DNA and Total RNA cultured cells and animal tissues using silica spin columns
  • Simple extraction process with no need for phenol or chloroform
  • High-quality extracted genomic DNA and RNA can be used for a range of downstream applications such as genomic sequencing, PCR, real-time qPCR, or Southern blotting

 

SKU List: #NK080-5, #NK080-50, #NK080-200

  • Materials Safety Data Sheets (MSDS)

    Lysis Buffer LB-DR - MSDS

    Wash Buffer WB-GD1 - MSDS

    Wash Buffer WB-GD2 - MSDS

    Wash Buffer WB-TR1 - MSDS

    Wash Buffer WB-R2 - MSDS

    Elution Buffer EB- MSDS

    Nuclease-Free Water - MSDS

     

  • Product Specifications

    Kit Type: DNA/RNA Extraction/Purification
    Kit Format: Silica Spin Columns
    Kit Scale: MINI
    Starting Material: Up to 1X10^7 cells; up to 30 mg tissue
    Processing Time:  < 40 minutes
    Typical Yield up to 100 µg for RNA and DNA
    Elution Volume:  50 - 100 μL
    Typical Ratio A260/A280: 1.7 - 1.9 for DNA; 2.0 - 2.2 for RNA
    Typical Ratio A260/A230: 2.0 - 2.3 for RNA and DNA
    Downstream Applications: Ligation, microarray, qPCR, RT-qPCR, Northern blot, poly-A purification, etc.

     

C$45.00Price

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