PuroMAG™ Total RNA Purification Kit

The PuroMAG™ Total RNA Purification Kit is designed to purify total RNA from bacteria, mammalian cells, yeast, or animal tissue. It greatly simplifies the extraction process by incorporating a convenient and easy-to-use extraction technology based on nucleic acid binding to silica-coated magnetic beads.
 
This kit includes DNase I digestion step to remove any contaminating DNA.
 
This kit can be used with a manual magnetic rack or automated purification systems. The high-throughput protocols have been optimized for Auto-Pure Nucleic Acid Purification systems, but can be adapted for other purification systems (i.e. Kingfisher Flex, Hamilton Microlab STAR, Biomek FX, etc.). The kit can also be used with our PuroMAGTM 8-Position Mini Magnetic Rack (Cat# MGR-008) and PuroMAGTM16-Position Mini Magnetic Rack (Cat# MGR-016) for fast and convenient manual purification of RNA. This kit can also be used to perform an RNA cleanup after DNase I treatment or other enzymatic treatments. High quality RNA extracted with the PuroMAG™ Total RNA Purification Kit can be used for a range of downstream applications such as reverse-transcription qPCR, Northern blotting, or microarray analysis.
 
Features:
Easily extract RNA from a variety of samples, with specialized protocols for each sample type 
Flexible magnetic bead-based protocols (low sample manual purification or high through-put automatic purification) and centrifugation is not required
Simple extraction process with no need for phenol or chloroform 
Includes on-bead DNase I digestion step to remove contaminating DNA
High quality extracted RNA can be used for a range of downstream applications such as cDNA library construction, microarray analyses, Northern blotting, qPCR, and RT-qPCR
 
Citations:
Kundu, Biki Bapi, et al. "Extracellular Respiration is a Latent Energy Metabolism in Escherichia coli." bioRxiv (2024): 2024-05.
 
SKU List: NKM051-96, NKM051-384