PuroMAG™ Protein A Coated Magnetic Beads #MGB-PRA-1, MGB-PRA-5

PuroMAG™ Prpotein A coated magnetic beads are designed for efficient capture of IgG and other antibodies from serum, cells, and other biological samples.
 
Protein A coupled PuroMAG  superparamagnetic beads are suitable for fast and efficient capture of a wide range of Ig’s. Protein A shows varying affinity for Ig’s from different species and for different isotypes within a species (see the Table in the Info section).
 
During the purification the sample containign Ig's is first mixed with PuroMAG Protein A coated beads. During 30 min incubation the Ig’s bind to PuroMAG-PRA beads via their Fc region. The samples are then placed on a magnetic rack for pull-down and washhing to produce a purified compelex of antibodies bound to Protein A beads. Bound antibodies can then be covalently cross linked to bead-immobilized Protein A to get a very stable bead-antibody complex for further antigen capture experiments.
 
Strong magnetic core allows PuroMAG™ Magnetic Beads to be separated out of solution with magnets in under 30 seconds. This allows for easy processing with magnetic racks, removing the need for a centrifuge.
 
PuroMAG™ Magnetic Bead technology can also be used with automated extraction systems for high throughput nucleic acid purification.
 
In Luna Nanotech we use the power of nanotechnology to optimize and improve the quality and surface binding capacity of nano and micromaterials. Try our magnetic beads and we are confident that they will outperform our competitors!
 
Features:
High binding capacity of Antibody:
IgG binding capacity: 44-50 µg/100µL beads
Rapid and efficient IgG purification from complex samples.
Fast response to a magnet.
High monodispersity.
 
Applications:
Magnetic separation of antibodies. The protein A coated magnetic beads are simply added to antibodies for binding and capture.
During the incubation time, the Ab binds to the MGB-PRA beads via their Fc-region. The samples are transferred to a magnetic rack for easy removal of unbound residue. 
The bead-bound Ab can be used for immunoprecipitation. 
 
SKU: MGB-PRA-1, MGB-PRA-5