PuroMAG™ Endo-Free Plasmid MIDIprep Kit #NKEM112-20, #NKEM112-50

The PuroMAG™ Endo-Free Plasmid MIDIprep Kit is designed to provide a simple and easy-to-use method for large-scale extraction and purification of endotoxin-free plasmid DNA from cultured bacterial cells.
 
This purification technology is based on plasmid binding to silica-coated magnetic beads, which allows for efficient removal of genomic DNA, protein, salt, and other contaminants. Following the lysis steps, endotoxins are removed using the Endotoxin Removal Buffer ERB.
 
Endo-Free Plasmid MIDIprep Kit can be used to purify plasmid DNA from up to 100 mL of overnight bacterial culture. PuroMAG™ Endo-Free Plasmid MIDIprep Kit can purify up to 200 µg of plasmid DNA while keeping endotoxin levels at <0.1 EU/µg.
 
The PuroMAG™ buffer formulations produce highly purified plasmid DNA for downstream applications, such as transfection, enzymatic digestion, or PCR. 
 
Features:
Purify endotoxin-free plasmid DNA from large volumes (up to 100 mL) of bacterial cultures
Flexible magnetic bead-based protocols (low sample manual purification or high through-put automatic purification) and centrifugation is not required
Optimized buffer formulations to ensure efficient removal of RNA from plasmid samples
Rapid  purification of plasmid DNA (< 60 minutes), compared to ion-exchange column formats (> 3 hours)
High quality and low endotoxin level (<0.1 EU/µg) extracted plasmid DNA can be used for a range of downstream applications such as cloning, PCR, restriction enzyme digest, sequencing, transformation, or transfection
 
SKU List: NKEM112-20, NKEM112-50