Protein A, Recombinant, Lyophilized

Protein A is a cell wall component produced by a number of strains of Staphylococcus aureus. It consists of a single polypeptide chain with few or no carbohydrate. Recombinant Protein A is produced in E. coli and in the same manner as native Protein A. The Protein A molecule contains four high-affinity binding sites that can interact with the Fc region from IgG of a number of species. Optimal binding occurs at pH 8.2, but binding is also effective at physiological conditions (pH 7.0 to 7.6).
Protein A can be coupled to a wide variety of reporter molecules including fluorescent dyes, enzyme markers, biotin, colloidal gold and radioactive iodine without affecting the antibody binding site. Protein A can also be immobilized on a solid support, such as agarose or acrylic beads, to purify immunoglobulins / antibodies, or used in immunoprecipitation assays.
 
Applications
Luna Nanotech Protein A is provided for research laboratory or bioprocessing purposes only. Not for use in diagnostic or theraputic human or animal applications.
 
Formulation
Lyophilized without additives and carrier free.
 
Purity
≥97% by SDS PAGE and HPLC analysis.
 
Source
Escherichia coli.
 
Storage
Store at 4°C if entire vial with lyophilized product will be used within 5 weeks. Store, frozen at -20°C . After reconstitution in distilled water or buffer, aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.