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Protein A, Fluorescent Dye Labeled

Protein A, Fluorescent Dye Labeled

Recombinant Protein A that has been covalently labeled with a fluorescent dye.

 

The following fluorescence dye labels are available:

  • AFDye 488
  • AFDye 555
  • AFDye 647
  • IR750

 

  • NOTE 1: AFDye 488 (similar to FITC), AFDye 555 (similar to Cy3), AFDye 647 (similar to Cy5) are analogs of AlexaFluor® 488, AlexaFluor® 555, and AlexaFluor® 647 dyes, respectively.
  • NOTE 2: IR750 dye is ithe analog of the IRDye® 750 dye.

 

Protein A is a cell wall component produced by a number of strains of Staphylococcus aureus. It consists of a single polypeptide chain with few or no carbohydrate. Recombinant Protein A is produced in E. coli and in the same manner as native Protein A. The Protein A molecule contains four high-affinity binding sites that can interact with the Fc region from IgG of a number of species. Optimal binding occurs at pH 8.2, but binding is also effective at physiological conditions (pH 7.0 to 7.6).

 

Applications

Luna Nanotech Protein A is provided for research laboratory or bioprocessing purposes only. Not for use in diagnostic or theraputic human or animal applications.

 

Formulation

Lyophilized powder

 

Storage

Store at 4°C if entire vial with lyophilized product will be used within 5 weeks. Store, frozen at -20°C . After reconstitution in distilled water or buffer, aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

  • Ig binding strength of protein A

    Ig Species / Subtype

    Affinity for Protein A

    Human IgG

    +++

    Human IgG1

    +++

    Human IgG2

    +++

    Human IgG3

    +

    Human IgG4

    +++

    Human IgM

    +

    Human IgD

    -

    Human IgA1

    +

    Human IgA2

    +

    Human Fab

    +

    Human scFv

    +

    Mouse IgG

    +++

    Mouse IgM

    -

    Mouse IgG1

    +

    Mouse IgG2a

    +++

    Mouse IgG2b

    +++

    Mouse IgG3

    +++

    Rat IgG

    +

    Rat IgG1

    +

    Rat IgG2a

    -

    Rat IgG2b

    -

    Rat IgG2c

    +++

    Rabbit IgG

    +++

    Goat IgG

    +

    Sheep IgG

    +

    Guinea pig IgG

    +++

    Hamster IgG

    ++

    Donkey IgG

    ++

    Pig IgG

    +++

    Dog IgG

    +++

    Cat IgG

    +++

    Chicken IgG

    -

    Cow IgG

    +

    Horse IgG

    +

  • Product Data Sheet

C$200.00Price

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