Mini-Beadbeater-96
The Mini-Beadbeater-96 is a high-energy, high-throughput cell disrupter. It can process one 2 ml deep well microplate (capacity 96 sample capacity), two 1 ml deep well microplates (192 sample capacity), or using an included 45 capacity polypropylene microvial rack, 0.6, 1.5, or 2 ml microvials.
The Mini-Beadbeater-96 rapidly disrupts cells and tissue by violently shaking samples inside microvials or standard deep well microplates partially filled with tiny ceramic or steel beads. Complete cell disruption is achieved in 1 to 3 minutes of beadbeating.
Even resistant samples like yeast, spores or fibrous tissue are completely homogenized 2- 3 minutes in 0.1 to 1 ml of extraction medium. The non-foaming, aerosol-free method preserves enzymes and organelles. In the presence of nucleic acid extraction media such as phenol, Gu-SCN, or a commercial kit solution, DNA and RNA are recovered in the highest possible yield. The method is ideal for PAGE, PCR applications and diagnostics using antibody or oligonucleotide probes. Because beads and microvials are disposable, there are no cross-contamination concerns when homogenizing multiple samples.
The Mini-Beadbeater-96 is also used for dry grinding. Steel beads are added to hard samples such as hair, bone, teeth, seeds, and minerals and are completely powdered in 10-60 seconds. Softer materials such as biological tissue, rubber, or plastics can also be powdered by first freezing the sample to liqN2 temperatures -- a process called Cryo-grinding.
Features:
Use a separate tube for each sample homogenization to avoid sample cross-contamination.
Can lyse most samples and preserve the integrity of biomolecules (DNA, RNA, proteins) and drug molecules
Disruption beads are available in a variety of material and sizes to suite the disruption of different sample types.
Bead homogenizer removes the need for nitrogen pre-treatment of the samples.
SPECIFICATIONS :
- Sample Capacity: Holds one 2 ml deep-well microplate or two 1 ml deep-well microplates. Also included is a microplate shaped vial holder wich can hold 4 to 45 microvials (0.5, 1.5 & 2.0 ml) each containing up to 400 mg (wet weight) of biomaterial.
- Vial displacement distance or Throw: 1.25 in. (3.2 cm).
- Shaking Speed: Variable speed. 1400 rpm to 2400 rpm (40 oscillations/second). Disruption efficiency has been engineered for equivalency with other MiniBeadbeater models having smaller vial capacities - thus developed protocols are interchangeable. The "calculated M/sec performance" value is greater than competitive beadbeater-type cell disrupters on the market.
- 3-D shaking pattern: Vial contents shake from the top to bottom of the vial or plate in a compressed figure-8 pattern. Vials and microplates are held in a near-horizontal orientation rather than the less efficient vertical position. This exclusive shaking pattern assures complete cell disruption in the shortest possible time. No reorientation of plates in mid-cycle is required.
- Timer: 0 - 5 minutes with automatic reset.
- Recycle delay time: No motor cool-down time is required during or between sample runs.
- Dimensions: Width: 16 in, Depth: 24 in, Height: 18 in, 120 pounds
- Maintenance: None